Establishment of a duplex fluorescent quantitative PCR assay for detection of Porcine circovirus type 2 and type 3

To establish a method for simultaneous detection of porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3), specific primers and TaqMan probes were designed after sequence alignment according to the specific sequences of PCV2 Cap gene and PCV3 Cap gene on GenBank. By optimizing the reaction conditions, a duplex fluorescence quantitative PCR detection method for simultaneous detection of porcine circovirus type 2 and 3 was established, and the specificity, sensitivity, and reproducibility were tested. Specificity test results showed that in addition to the positive test results for PCV2 and PCV3, tests for PRRSV, CSFV, PPV, PRV, PEDV, and TGEV were all negative with no cross-reaction, indicating its good specificity. Sensitivity test results showed that the minimum detection limit for detection of PCV2 and PCV3 can both reach 10 copies.L-1, indicating its high sensitivity. The coefficient of variation within and between groups of this method was less than 2%, indicating its good stability. A total of 181 pork and whole blood samples collected from Zhejiang Province were tested using the detection method established in this article and the standard common fluorescent PCR detection method. The results showed that the positive rate of PCV2 was 50.83% (92/181), the positive rate of PCV3 was 37.57% (68/181), and the co-infection rate of PCV2 and PCV3 was 12.15% (22/181). The above detection results of ordinary fluorescent PCR were 50.28% (91/181), 36.46% (66/181), and the co-infection rate was 11.60% (21/181). The coincidence rates of the two methods for PCV2 and PCV3 can reach 98.91% and 97.06%, and the coincidence rate for PCV2 and PCV3 mixed infection were 95.45%. In summary, the duplex fluorescence quantitative PCR detection method established in this experiment can distinguish PCV2 and PCV3 rapidly, which can be used for pathogen detection and epidemiological investigation.

Effects of Porcine deltacoronavirus on the transduction pathway of the innate immune signal

Porcine deltacoronavirus (PDCOV) is a new type of pig intestinal coronavirus, which targets pig small intestinal epithelial cells to cause severe enteritis. After infecting the host, PDCoV finishes its proliferation in the host cell by antagonism or escape the innate immune signaling transduction pathway. In order to understand the action mechanism of PDCOV 0n the congenital immune signal transduction pathways, this paper reviews the effects of PDCOV on RLR, Jak-STAT, MAPK and mitochondrial signaling pathway to clarify the relationship between PDCOV and host innate immune signaling transduction pathways in order to provide help for the prevention and treatment of PDCOV infection.

SUS funding in the face of the Covid-19 pandemic

The Brazilian Unified Health System (SUS), since its creation in 1988, has a history of insufficient resources to effectively guarantee universality and comprehensive care. Its underfunding, derived from the lack of support from part of society and neoliberal policies undertaken by various governments in the 1990s and 2000s, turned into effective reduction in real terms of funding resources after the approval of the "expenditure ceiling” in 2016, aggravating the structural system difficulty. The Covid-19 pandemic generated a preeminent need to expand the service capacity of the public health system and, with that, the resources for the SUS were expanded. However, this greater financial availability was the result of extraordinary measures and was restricted to the years 2020 and 2021. The ordinary budget for health in those years and the 2022 LOA demonstrate that the underfunding continues and that the pandemic did not change the lack of priority given to healthcare within the federal budget. © 2023, Universidade de Sao Paulo. Museu de Zoologia. All rights reserved.

Effect of Drebrin protein on the proliferation of PEDV in Vero cells

Previous studies have revealed that developmental regulated brain protein (Drebrin) is involved in cell- to-cell communication, nerve transmission, tumor metastasis, spermatogenesis and other life activities, but there are few studies on viruses. The aim of the current research was therefore, to study the function of Drebrin and its effect on the proliferation of porcine epidemic diarrhea virus (PEDV). The Drebrin gene was cloned according to the Drebrin gene sequence (XM_008015438.2) of Chlorocebus sabaeus registered by GenBank, and the phylogenetic tree was constructed to analyze its homology. The results showed that the CDS region of Vero cells Drebrin gene was 2088 bp long, encoding 695 amino acids, and was relatively conserved and had high homology with all species. To investigate the effect of Drebrin on the proliferation of PEDV in Vero cells, the eukaryotic expression vector pcDNA3.1-Drebrin-Flag was constructed. After transfection of Vero cells with different concentrations of pcDNA3.1-Drebrin-Flag, cells were infected with PEDV. Our results showed that overexpression of Drebrin in Vero cells could significantly inhibit the intracellular PEDV mRNA level and N protein expression, reduce the extracellular virus titer and inhibit the proliferation of PEDV. Further study on the interaction between Drebrin and PEDV S proteins by laser confocal technique was also performed. The results showed that Drebrin and S protein were co-located in the cytoplasm, suggesting that the two proteins may interact with each other. This study demonstrated for the first time that Drebrin can inhibit PEDV proliferation in Vero cells, laying a foundation for further research in to Drebrin function and provides a valuable information for anti-PEDV research.

Prototype of a Device for the Automatic Measurement of Physiological Signals to Assist the Diagnosis and Monitoring of Patients with Covid-19

This article describes the design, construction and preliminary results of a device to automate the mea-surement of physiological signals to assist in the diag-nosis and monitoring of COVID-19. The device uses a system to controlling linear actuators to turn on/off certified biomedical instruments, in addition to algo-rithms for image recognition of displays with measure-ments of temperature, oxygen saturation, pressure and heart rate. The system also includes a mobile ap-plication, which receives data in real time and creates a database for medical evaluation. Results obtained with the device have demonstrated to provide a high percentage of efficiency in the data acquisition. After several trials with users, SUS and PSSUQ tests were applied to allow verifying the users' feedback regard-ing the satisfaction and usability of the prototype, with high score, showing the good acceptance of the device from the users.

Development and application of a multiplex fluorescent quantitative RT-PCR for detection of Porcine epidemic diarrhea virus, porcine coronavirus and porcine acute diarrhea syndrome coronavirus

To develop a multiplex fluorescent quantitative RT-PCR for the detection of porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and swine acute diarrhea syndrome coronavirus (SADS-CoV), in this study, specific primers/probes were designed based on the conserved regions of M, M and N gene sequences of PEDV, PDCoV and SADS-CoV, respectively. After optimization of the reaction conditions, a multiplex fluorescent quantitative RT-PCR for PEDV, PDCoV and SADS-CoV was established. The results of specificity assay showed that the method was positive for detection of PEDV, PDCoV and SADS-CoV, and negative for detection of porcine transmissible gastroenteritis virus, porcine rotavirus, porcine reproductive and respiratory syndrome virus, porcine pseudorabies virus, porcine circovirus type 2, porcine parvovirus, classical swine fever virus and foot-and-mouth disease virus. The results of sensitivity assay showed that the detection limit of this method for PEDV, PDCoV, and SADS-CoV plasmids standard was 1.0x101 copies/L, and had a good linear relationship with their Ct values in the range of 101 copies/L to 106 copies/L. The results of repeatability assay showed that the coefficients of variation (CVs) of intra- and inter-assay reproducibility ranged from 0.33% to 2.53%, indicating good repeatability and stability. To evaluate the effects of the developed method, 100 clinical samples collected from different parts of Henan province were used for detection of these three viruses and compared with those of single RT-PCR and standard methods. The results of multiplex fluorescent quantitative RT-PCR showed that the positive rates of PEDV, PDCoV and SADS-CoV were 38% (38/100), 14% (14/100) and 5% (5/100), respectively. There was no mixed infection. The coincidence rate with the standard detection methods of PEDV and PDCoV was 100%, and the sensitivity was higher than that of single RT-PCR. In this study, a specific, sensitive and rapid multiplex fluorescent quantitative RTPCR method was established for the first time, which could be used for the differential detection of PEDV, PDCoV and SADS-CoV, and laid a foundation for the differential diagnosis and control of porcine diarrheal diseases.

Game meat procurement market in Poland in 2009-2021

Procurement of game animals is a major source of revenue for hunting clubs in Poland. For several years, the game meat buying market has been showing an upward trend, but this situation is also influenced by random factors that negatively affect the value of the game meat buying market. For several years in our country we have been struggling with the ASF virus, and since 2020, negative effects in the economy related to the occurrence of the SARS-CoV virus have been observed, also affecting the hunting sector with its activities. The aim of the study was to analyze the dynamics of game meat procurement in Poland in the years 2009-2021. The data concerned the three most important species, namely deer, roe deer and wild boar. The analysis covered the quantity of game meat, procurement value and the average price of game meat depending on animal species. The conducted research confirmed an upward trend in the volume and value of game meat procurement for all the analysed game species. Similarly, the average procurement prices of roe dee and wild boar meat with the exception of red deer, showed an upward trend. The study confirmed the negative impact of the ASF virus and the SARS-CoV-2 virus on the game meat buying market in Poland.

Crise sanitária e atenção primária: a atuação de assistentes sociais nos territórios

Este artigo focaliza a atuação de assistentes sociais na atenção primária em saúde (APS), na pandemia do Covid-19. Discute a crise sanitária, problematizando a ofensiva ultraneoliberal e suas implicações na atenção primária em saúde cuja potencialidade assistencial foi esvaziada por meio de várias medidas tomadas pelo Governo Federal, como mudanças na Política Nacional de Atenção Básica e o Previne Brasil. Foi realizada pesquisa nos Anais do IX Congresso Nacional de Serviço Social em Saúde, destacando trabalhos sobre APS. Foi desenvolvida análise com base no materialismo-histórico-dialético, considerando a historicidade, mediações e contradições. Foram identificados desafios à atuação profissional, como o uso de Tecnologias de Informação e Comunicação (TIC), o teletrabalho, a precarização da política de saúde. Entre as estratégias de ação utilizadas destacam-se: ações educativas, articulação com a rede socioassistencial, entre outras. Considera-se que a negação de direitos como método governamental distancia o horizonte emancipatório do projeto ético-político da profissão.Alternate :The article focuses on the role of social workers in primary health care (PHC) during the covid-19 pandemic. It discusses the health crisis, questioning the ultra-neoliberal offensive and its implications for primary health care, which its care potential has been emptied, through various measures taken by the Federal Government, such as changes in the National Primary Care Policy and Previne Brasil. Research was carried out in the Annals of the IX National Congress of Social Service in Health, highlighting works on PHC. An analysis was developed based on dialectical-historical-materialism, considering historicity, mediations and contradictions. Challenges to professional performance were identified, such as the use of Information and Communication Technologies (ICT), teleworking, and the precariousness of health policy. Among the action strategies used, the following stand out: educational actions, articulation with the social assistance network, among others. It is considered that the denial of rights as a governmental method distances the emancipatory horizon from the ethical-political project of the profession.

The re-parameterized inverse Gaussian regression to model length of stay of COVID-19 patients in the public health care system of Piracicaba, Brazil.

Among the models applied to analyze survival data, a standout is the inverse Gaussian distribution, which belongs to the class of models to analyze positive asymmetric data. However, the variance of this distribution depends on two parameters, which prevents establishing a functional relation with a linear predictor when the assumption of constant variance does not hold. In this context, the aim of this paper is to re-parameterize the inverse Gaussian distribution to enable establishing an association between a linear predictor and the variance. We propose deviance residuals to verify the model assumptions. Some simulations indicate that the distribution of these residuals approaches the standard normal distribution and the mean squared errors of the estimators are small for large samples. Further, we fit the new model to hospitalization times of COVID-19 patients in Piracicaba (Brazil) which indicates that men spend more time hospitalized than women, and this pattern is more pronounced for individuals older than 60 years. The re-parameterized inverse Gaussian model proved to be a good alternative to analyze censored data with non-constant variance.

Glycyrrhizin inhibits swine acute diarrhea syndrome coronavirus infection via the HMGB1/TLR4/JNK signal pathway

Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly discovered enteric coronavirus, is the etiological agent that causes severe clinical diarrhea and intestinal pathological damage in piglets. In this study, Vero E6 and IPI-2I cells were pretreated with different concentrations of glycyrrhizin (GLY) for 2 hours, and then infected with different concentrations of SADSCoV, aiming to investigate the inhibitory effect of GLY on SADS-CoV. Western blot and TCID50 results revealed a significantly decreased N protein expression and viral titer, indicating that GLY can inhibit the infection of SADS-CoV. Vero E6 and IPI-2I cells were pretreated with different concentrations of GLY for 2 hours and infected with SADS-CoV. Western blot results showed that when the concentration of GLY was 0.8 mmol/L, the expression of N protein decreased significantly, indicating that GLY inhibited the invasion of the virus. At first, cells were treated with 0.4 mmol/L GLY, and cell samples were collected at 2 hours, 6 hours and 12 hours after being infected with SADS-CoV for analysis, and the expression of N protein were found to be significantly reduced at all points, indicating that GLY had a significant inhibitory effect on the replication of the virus. GLY is a competitive inhibitor of high mobility group box 1 (HMGB1), and the receptors of HMGB1 mainly include TLR4 and RAGE. Based on this fact, the mutant plasmid at the key sites of HMGB1 (C45S, C106S, C45/106S) and the siRNA of the RAGE receptor were transfected to Vero E6 cells and infected with SADS-CoV, and the cell supernatant and samples were harvested. The western blot and TCID50 results showed that the expression of N protein and the virus titer were decreased, suggesting that GLY exerts its function by affecting the binding of HMGB1/TLR4/RAGE during SADS-CoV infection. To further explore the signaling pathway through which GLY functions, Vero E6 and IPI-2I cells were inoculated with SADS-CoV, and cell samples were harvested, western blot was used to detect the changes of MAPK proteins. The results showed that the protein expression levels of p-p38, p-JNK and p-ERK were up-regulated in the early and late stages, indicating that the MAPK pathway was activated by SADS-CoV infection. Vero E6 and IPI-2I were pretreated with different concentrations of GLY and TLR4 inhibitor TAK for 2 hours and infected with SADS-CoV. Protein samples were harvested and analysed by western blot which showed a decreased p-JNK and N proteins, while other proteins showed no significant changes. These results indicated that GLY and TAK regulated the phosphorylation of JNK but did not regulate the phosphorylation of p38 and ERK. Also, Vero E6 cells were treated with HMGB1 antibody, the siRNA of HMGB1 and HMGB1 mutants plasmid, and infected with SADS-CoV. Protein samples were harvested, western blot results showed that phosphorylation of JNK decreased, indicating that HMGB1 affected JNK phosphorylation. Finally, Vero E6 and IPI-2I cells were pretreated with different concentrations of JNK inhibitor SP600125 to infect SADS-CoV, western blot, TCID50 and IFA results showed that the expression of N protein and virus titer, as well as virus replication were reduced, indicating that SP600125 inhibited virus replication. In conclusion, our results revealed that GLY can inhibit in vitro replication of SADS- CoV, mainly through the HMGB1/TLR4/JNK signaling pathway. The discovery of this pathway provides theoretical support for the research of novel anti-SADS-CoV drugs.

Swine breeding in the villages of Valcea county, Oltenia (Romania) - tradition or necessity?

Food supply has been a constant source of concern for mankind. In the present context, with food security a priority of European and national policies, an analysis of pig farming in a representative NUTS2 administrative level of Romania that emphasizes the proportion of households raising at least one pig and the main factors influencing farmers to adopt or give up swine breeding could allow a much clearer understanding of this phenomenon that lies at the border between cultural tradition and socio-economic necessity. This study uses mixed methods that complement each another to help reveal this complex phenomenon in the analyzed territory. Cluster analysis shows the concentration of swine breeding and maps its spread in terms of both subsistence and larger farms, and qualitative interviews prove the motivation of farmers to continue in this occupation. As a primary result, the study visualizes the spatial distribution of pig farming in the rural environment of Valcea county, Romania, from a diachronic perspective in the post-communist period. It also reveals areas of differing concentrations of both very small-sized farms, which prioritize meeting their own food needs, and larger farms, which prioritize commercial production to supplement their revenue streams. Both categories, but particularly the latter, are of particular interest in a period in which the socio-economic environment after 1990 - marked by economic restructuring, unemployment, population migration, the economic crisis of 2008-2010, the pandemic of 2020-2021, and the most recent energy crisis - periodically highlights the importance of rural areas in ensuring food security and sufficiency at both the local and regional levels.

Interaction between target peptide and complementary peptide by bimolecular fluorescence complementary technology

[Objective] Using the bimolecular fluorescence complementation (BiFC) technology, the present experiment aimed to study the interaction relationship and localization of the target peptide and the complementary peptide based on the porcine epidemic diarrhea virus (PEDV) S protein receptor binding site peptide in living cells, so as to provide the foundation and theoretical support for the further use of the peptide in the detection of porcine epidemic diarrhea virus. [Method] The target peptide was designed according to the physical and chemical characteristics of the target protein, such as the amino acid composition, the type of charge, the ability to form intennolecular hydrogen bonds, the strength of polarity, and hydrophobicity;According to the amino acid composition of the target protein, a complementary peptide that interacted with it in theory was designed, and the target peptide and complementary peptide were predicted and analyzed by using bioinfonnatics tools;The target peptide and complementary peptide were inserted into the pBiFC-VC155 and pBiFC-VN173 vector, which was double digested by the EcoRI/XhoI and NotI/SalI, respectively, verified by enzyme digestion and sequencing, and then transfected into Vero cells to study the interaction between the target peptide and the complementary peptide, and the precise localization of BiFC complex in cells. [Result] Bioinfonnatics analysis showed that the target peptide and complementary peptide had hydrophilic and hydrophobic domains, respectively, and the hydrophilic domains were both positively and negatively charged, which could generate electrostatic attraction. The results of enzyme digestion and sequencing showed that the pBiFC-VC155-target peptide and pBiFC-VNI73-complementary peptide plasmids were successfully constructed;Cell transfection experiments showed that the target peptide and complementary peptide could form BiFC complexes in Vcro cells after co-transfection of recombinant plasmids, indicating that they could interact with each other;Indirect immuttolluorescence assay confirmed that the BiFC complex was mainly distributed in the nucleus. [Conclusion] It was confirmed that the peptide designed based on the PEW/ S protein receptor binding site can interact with each other in living cells, demonstrating the feasibility of the peptide for detection.

Improved virus inhibition by microencapsulated monoclonal antibody against porcine epidemic diarrhea virus

Porcine epidemic diarrhea virus (PEDV) has been affecting the swine industry, especially in suckling pigs in with a high mortality rate. Among all the strategies to overcome PEDV, boosting mucosal immunity in pig intestine via oral administration appears to be more efficient than other routes. However, there are biological obstacles such as acidic environment that could damage biologics, a product from organisms often used for PEDV treatment. The plant-derived 2C10 monoclonal antibody (mAb) from Nicotiana benthamiana produced by transient expression was revealed as one of the potential candidates against PEDV through oral delivery. Herein, we demonstrated the calcium-alginate microencapsulation system to protect the 2C10 mAb from the harsh condition in the stomach and to be released the 2C10 mAb when arriving in the intestine. The pH-responsive encapsulated 2C10 mAb microbeads were constructed from the calcium-alginate system. The microbeads were well-tolerated under the acidic environment of simulated gastric fluid (SGF) and were digested under the alkaline condition of simulated intestinal fluid (SIF). The encapsulated 2C10 mAb in the SPF-treated microbeads exhibited high virus neutralization efficiency in Vero cells when compared to the unencapsulated 2C10 mAb treated by SPF that cannot neutralize the virus. For these reasons, calcium-alginate microencapsulation system is an attractive platform to be considered as a candidate for the next generation of oral vaccine development.

Duplex Taq man qPCR for detecting porcine epidemic diarrhea and transmissible gastroenteritis viruses and epidemic study in Fujian

Objective: A Taq Man probe-based duplex real-time PCR for rapid detection of porcine epidemic diarrhea virus(PEDV) and transmissible gastroenteritis virus(TGEV) was developed. A study was conducted using the methodology to analyze the related 2019-2021 epidemic occurred in Fujian. Method: Specific primers and probes labeled with FAM and VIC were designed to amplify the N gene of PEDV and the S gene of TGEV, respectively. A reaction system for the assay was established, optimized, and tested for sensitivity, specificity, and repeatability. The assay was used for the viral detection on297 suspected clinic specimens collected from 2019 to 2021 for an epidemiology study. Result: The newly developed duplex qPCR methodology showed a sensitivity of 10 copies.L-1 on PEDV and TGEV, which was 100 times higher than that of regular PCR. There were no cross reactions with other common viruses. The inter-and intra-assays had variations on Ct values below 1%. On the 297 specimens, the infection rate of PEDV was 88.89%, that of TGEV 1.01%, and that of both PEDV and TGEV 3.37%. Conclusion: The established duplex qPCR had high sensitivity, specificity, repeatability, and reproducibility for detecting PEDV and TGEV. The 2019-2021 epidemic involving the viruses appeared to be mostly PEDV with low incidents of mixed TGEV and PEDV/TGEV infection.

Factors related to tertiary education teachers' use of the Microsoft Teams platform

Previous research disclosed that teachers faced challenges while using different learning management systems during the COVID-19 outbreak. This study investigated teachers' perceptions of Microsoft Teams' functionalities and the extent to which they are related to teachers' time spent on it as well as the various factors associated with their syllabus coverage. Situated within human-computer interaction theory, 157 university teachers at Chouaib Doukkali University, Morocco, were administered a questionnaire that measured their perception of the functionalities offered by Microsoft Teams. Results indicated that teachers' perceptions of the functionalities offered by learning management systems also play an intervening variable in the deployment of these tools in teaching. Hence, this paper calls for an extension and re-adaptation of existing models such as the technology acceptance model and the system usability scale to include an evaluation of the functionalities offered by these tools.

Infectious disease Roundup 2022

This paper describes the epidemiology, prevalence, transmission, prevention and control of some infectious diseases in companion animals, livestock, wild animals and humans in Ontario, Canada, in 2022, including SARS-CoV-2;Echinococcus multilocularis, Leishmania spp. and SARS-CoV-2;antimicrobial stewardship resources;2 cases of rabid dogs imported from Iran (July 2021 and January 2022);prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriacea, Dirofilaria immitis, Brucella canis, canine parainfluenza and adeno- and herpes viruses in dogs recently imported from Asia;Paragonimus kellicotti lung flukes and Streptococcus equi subsp. zooepidemicus in dogs;African swine fever in pet pigs, backyard pigs and wild pigs and blastomycosis in dogs and humans.

Factors related to tertiary education teachers' use of the Microsoft Teams platform

Previous research disclosed that teachers faced challenges while using different learning management systems during the COVID-19 outbreak. This study investigated teachers' perceptions of Microsoft Teams' functionalities and the extent to which they are related to teachers' time spent on it as well as the various factors associated with their syllabus coverage. Situated within human-computer interaction theory, 157 university teachers at Chouaib Doukkali University, Morocco, were administered a questionnaire that measured their perception of the functionalities offered by Microsoft Teams. Results indicated that teachers' perceptions of the functionalities offered by learning management systems also play an intervening variable in the deployment of these tools in teaching. Hence, this paper calls for an extension and re-adaptation of existing models such as the technology acceptance model and the system usability scale to include an evaluation of the functionalities offered by these tools. Copyright © 2022 Inderscience Enterprises Ltd.

A Usability-focused Method to Design a Cybersecurity MOOC for Children

Teaching cybersecurity to children is not a trivial task due to the complexity of explaining online-threat-related topics such as sexting or cyberbullying. Moreover, most of the courses designed to raise awareness in these controversial subjects has been mainly produced in English. However, during the Covid-19 pandemic, getting access to Spanish-based training for protecting children online became a priority for parents in countries such as Ecuador, which was not prepared to have children studying at home whilst being exposed to such threats. In this paper, we show that it is feasible to design a virtual course (MOOC) to teach cybersecurity to Spanish-speaking children with acceptable usability levels and minor usability issues. Our proposed methodology combines ADDIE, DPIPE and UDL for developing the course, and uses both SUS and Nielsen as methods to assess the MOOC from an usability point of view. For enhancing user engagement, our course uses novel 3D anthropomorphic characters and storyboards to aid the learning process. Regarding usability results, our proposed MOOC has very high and acceptable scores, with around 86/100 using the SUS scale, and less than 3 in the Nielsen score. © 2022 IEEE.

Susceptibility of different animal species to experimental infection with SARS-CoV-2 (review)

The susceptibility of animal species to SARS-CoV-2, under experimental conditions, is a subject of great interest for the international scientific community. Compared to observational studies of natural disease outbreaks in different animal species, experimental studies based on in silico, in vitro and in vivo research, are important alternatives to evaluate the prediction of potential hosts for SARS-CoV-2 infection. In order to determine the susceptibility of a host species and the risk of acting as a potential animal reservoir, a large number of different animal species, domestic and wild, were experimentally infected with SARS-CoV-2, which were classified as permissive or resistant. Experimental infections have proven to be crucial for clarifying aspects of the pathogenetic mechanism, viral persistence and elimination, immune response, antiviral sensitivity, vaccine production, immunotherapy and improving diagnostic methods. In this article, some experimental infections carried out in different animal species will be reviewed, according to the data from the literature.

Automation of the IoT-Based COVID-19 Isolation Room Temperature and Humidity Control System at Telkom University

During the current COVID-19 pandemic, the large number of positive cases of infection has resulted in medical institutions lacking personnel to treat patients who continue to arrive. As a result of these problems, supervision and monitoring of room conditions is still lacking or even non-existent, so that the recovery process can be hampered or can facilitate the transmission of the virus to other people. It takes a device or tool that can monitor conditions and regulate the isolation room so that the temperature and humidity remain in the optimal zone so that recovery can be optimal and also reduce the risk of virus transmission. Based on this description, the author applies the concept of IoT by utilizing the IoT platform system and designing a system and tool that can monitor and regulate the COVID-19 isolation room and convey this information quickly and concisely. In addition, this study also examines how well and easily understood the system is when used by end-users by using the System Usability Scale or SUS as its usability testing method. The results obtained from this study are that the system and equipment function properly, the automation system and the method used are able to mitigate changes in temperature and humidity in the isolation room, and through the SUS method, the level of usability for end-users is deemed quite sufficient. © 2022 IEEE.